Live cell imaging or light sheet mode that exceeds a 4hour duration must be conducted at. No 159330033 vers 01082001 click on an item means to move the pointer onto the specified item and press and release the mouse button once. Leica tcs sp8 x the only freely tunable confocal system the white light laser source of the leica tcs sp8 x perfectly matches the spectral properties of any fluorophore excitable in the visible spectral range. Leica ssystem a class of its own leica s3 64 megapixel. Leica m typ 240 rangefinder digital camera sports a 24 mp fullframe cmos sensor delivers natural and brilliant color rendition and impressive reproduction of.
Tailor your microscope to your current needs and upgrade with additional functionalities at any time. The sp8 lightning allows you to perform proper sampling over a specimen area at least six times larger than with any other confocal system. User guide to the ibif leica tcs sp8 mp confocal microscope. Leica sp8 tcs users manual follow the procedure for start up and log on as posted in the lab. Pdf leica rm2255 ifu 2v3j en pdf leica rm2255 ifu 2v3j es. Leica sp8 the leica microsystems sp8 confocal with digital light sheet has the following features and capabilities. Double click on the las x software found on the desktop. Turn on the fluorescent lamp top left of the computer monitor. Page 1 user guide to the ibif leica tcs sp8 mp confocal microscope this version. They prefer to invest their idle time to talk or hang out. The leica sp8 5d is equipped with a series of five tuneable photoreceptors.
Leica biosystems division of leica microsystems inc. Leica tcs sp8 technical documentation en 11 2016, pdf, 499. The leica sp8 in the keck lab has additional hyd detectors, resonant scanner, live cell imaging mode and light sheet. Leica sp8 resonant confocal nrimcdb microscopy facility. Leica m9 m9p camera user manual, instruction manual, user guide pdf free download leica m9 m9p pdf user manual, user guide, instructions, leica m9 m9p owners manual. Leica sp8 pointscanning confocal system, on a leica dmi6000b inverted microscope, confocal laser scanning microscope leica tcs sp8 en, pdf, 535. Introduction the ibif confocal microscope is made available on a feeforusehour basis to all users who have been trained.
School of life sciences these are procedures provided by leica microsystems that describe how to operate the instrument in its most simplest format. May 18, 2016 leica m9 m9p camera user manual, instruction manual, user guide pdf free download leica m9 m9p pdf user manual, user guide, instructions, leica m9 m9p owners manual. Your investment in a leica tcs sp8 will pay off now and in the future. Upright leica dm6 cs leica dm6 cfs leica dm6 cfs wo tl axis inverted leica dmi8 cs.
The components are the microscope body, two automated stages, the scan head, the electrooptical modulator eom, the monitor and computer, and the spectraphysics maitai tunable nearir laser. Leica m typ 240 camera user manual, instruction manual, user guide pdf free download leica m typ 240 pdf user manual, user guide, instructions, leica m typ 240 owners manual. Never again compromise on speed, resolution and sensitivity. The gain setting as discussed elsewhere in this manual controls the sensitivity. Leica microsystems cms gmbh am friedensplatz 3 d68165 mannheim germany. Check to make sure antivibration table is on power switch in the back of unit 3. Operational training manual sp8 quick start instructions. Leica m9 m9p is the mmount fullformat digital rangefinder camera equipped with a 18 mp ccd sensor developed specifically for this camera, enables the capture of. Leica dm2500 upright fluorescent microscope manuals cell. Then turn on the power supply for the scanner 1 and the lasers 2 by. Solid state lasers and the el6000 widefield light source require only minimal warm up, but adhere to 15min15min rule. User manual sp8 leica confocal janvier2016 turn on the. This user manual provides you with important information for using the system, the necessary ambient conditions and the usable lasers.
Sp8 lightning konfokalmikroskop produkte leica microsystems. If you turn it on you will not be able to use slower speeds. The leica tcs sp8 is designed to meet all of your future requirements with effectiveness and efficiency. Leica sp8 white light confocal, gsted, flim cell sciences. Quick start leica tcs sp8 system start up image acquisition zseries acquisition system shut down software start up laser start up turn on the microscope control box. Turn the pc microscope, scanner power, laser power, and the laser emission key to on bottom right of desk. User manual leica tcs sp8 leica tcs sp8 mp leica tcs sp8 x. It is built around the leica prismbased spectral detector system on a motorized inverted microscope stand. Frap with resonant scanner appletter en, pdf, 2 mb. Leica tcs sp8 option machineposter en pdf, 728 kb sp8 scan head with 4tuneposter rgb may2018. User guide created by daniel desantis and linda mayernik, october, 2015. If you do not require high speed scanning leave resonant set to. This screen highlights the aperture opening and the intensity of the transmitted light. Leica sp8 tcs users manual v2 university of oklahoma.
Navigator software module creates a fast overview of samples. The microscope is suitable for both live cell and fixed specimens on slides, and it will run alongside the old zeiss lms 510 metahead confocal. Live cell imaging with adaptive focus control afc to automatically maintain focus in timelapse experiments. This user manual specifies names of products or services that are trademarks or registered.
Helene javot, sp5 confocal microscope user manual, updated by mamta srivastava 2 022012, page 1 15 pcic plant cell imaging center bti, room 104b confocal leica sp5 user manual startup procedure. Las x live data mode recording of manual and automated workflows, trigger functions, complex timelapse series. Leica tcs sp8 technical documentation life science research division. The leica sp8x smd confocal microscope is located in room 00075, lab 1. Turning on the system 1turn on the fluorescent light source number 1 2 turn on the ndd power supply. Training guide for leica sp8 confocalmultiphoton microscope. Instructions for using leica dcm3d confocal microscope. The instrument is a leica tcs sp8 mp confocal microscope, equipped for multiphoton confocal microscopy.
Leica sp8 confocal, sted, flim system microscopy and. This user manual specifies names of products or services that are trademarks or. It achieves this level of performance using highspeed resonant scanning with imaging formats of up to 2496 x 2496 pixels. The leica sp8 x smd confocal microscope is located in room 00075, lab 1. Quick operation manual for leica sp8 confocalmultiphoton microscope. A guide through the live data mode live data mode with leica tcs sp8 5 3 definition of an experiment some experiments can be more complex and require differ ent hardware settings at a certain time within the course of the experiment. The leica sp8 confocal microscope is for live cell imaging with highspeed, multispectral, supersensitivity, superresolution, and fluorescence lifetime capability.
In addition, this operating manual provides a rough overview of the operating principle of laser scanning microscopes. Database contains 3 leica dmi6000b manuals available for free online viewing or downloading in pdf. Turn on the ctr control box, el6000fluorescent light source for the. This user manual specifies names of products or services that. Sp8 lightning is a modular confocal microscope designed for optimal photon efficiency and high speed.
Sp8 lightning confocal microscope products leica microsystems. View and download leica tcs sp8 mp user manual online. Chinese, english, french, german, indonesian, italian, japanese, korean, polish, russian, taiwanese. Pdf leica rm2255 ifu 2v3j en pdf leica rm2255 ifu 2v3j es pdf leica rm2255 ifu 2v3j fr pdf leica rm2255 ifu 2v3j it pdf. Continuously tunable excitation and detection ensure best. Enable the lasers by turning the key from the off position. The leica microsystems sp8 confocal with digital light sheet has the following features and capabilities. The instrument is a leica tcs sp8 mp confocal microscope, equipped for multi photon confocal microscopy. The leica sp8 in the keck lab has additional hyd detectors, resonant scanner, live cell. Turn on power to microscope power unit is located at your feet, next to the computer. If you do not require high speed scanning leave resonant set to off. Adjust the laser intensity of the appropriate laser. The microscope is suitable for both live cell and fixed specimens on slides, and it will.
Turn on the scanner power 1 on the front of the compact supply unit csu 3. Adjust the laser intensity of the appropriate laser lines by moving the slider up or by directly. Unlike our previous instrument, it has a number of hardware features, specifically the objectives, the stage, and the condensers, that can be added or removed by the user, with the. Quick operation manual for leica sp8 confocalmultiphoton. Leica dm2500 upright fluorescent microscope manuals. Together with an aobs acoustical optical beam splitter up to 8 discrete laser lines can be selected simultaneously with a 1 nm precision. The portable document format pdf redundantly pdf format is a file format developed by adobe in the 1990s to present documents including text formatting and images in a manner independent of application software hardware and operating systems. Usermanual for leica sp8 confocal contact information.
Leica microsystems heidelberg gmbh lcs software functions user manual leica lcs english page 8 of 164 art. Turn on the ctr control box, fluorescent light for the microscope stand. View and download leica sp8 training manual online. User manual leica tcs sp8 smd for fcs, flim and flcs. Contents startup preparing for imaging part 1 on the scope. Sp8 lightning confocal microscope leica microsystems. Leica sp8 dive multiphoton microscope manualquick guide. For such experiments, live data mode is the tool of choice. In addition, this operating manual provides a rough overview of the.
Remove the dust cover from the microscope turn on the mercury lamp a if you need to see the fluorescence through the eye pieces. Microscopes microscope stand options upright leica dm6 cs leica dm6 cfs leica dm6 cfs wo tl axis inverted leica dmi8 cs leica dmi8 cel compact leica dmi8 cel advanced vibration isolation isolation options speci. Usermanual for leica sp8 confocal universitetet i bergen. User manuals, guides and specifications for your leica dmi6000b microscope. For information about the licenses offered, refer to this page.
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